Full Spectrum / Spectral Flow Cytometry
Instrument specifications
The Cytek Aurora leverages full spectrum technology with five lasers, three scattering channels, and 64 fluorescence channels to enable the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application.
• 67 detection channels (64 fluorescence channels,FSC, blue laser SSC, and violet laser SSC).
• 40 colors demonstrated, including fluorochromes with emission spectra in close proximity to each other.
• No need to reconfigure optical filters for different fluorochromes.
• Resolution of fluorochrome combinations with high spectral overlap.
• Automatic Micro-Sampling System (AMS) for acquisition of 96 well plates or 40-tube racks.
• Full spectrum cytometry enables efficient autofluorescence extraction.
• Exceptional small particle detection.
• SSC can resolve 0.2 µm beads from noise.
Five-laser configuration:
355 nm: (16 channels, 365-829 nm), UV
405 nm: (16 channels, 420-829 nm), Violet
488 nm: (14 channels, 498-829 nm), Blue
561 nm: (10 channels, 567-829 nm), Yellow-Green
640 nm: (8 channels, 652-829 nm), Red
Hands-on Training
To schedule a training session, please email the Flow Cytometry Core: flowcore@ku.edu
Training $/hour rates are available on the Rates page.
Cytek Aurora / Spectral Flow Cytometry Training Series
1. Introduction to Full Spectrum Flow Cytometry
5. Performing Spectral Unmixing
6. Steps for Setting Up a Panel
Spectral Panel Design and Optimization
Ferrer-Font L, Pellefigues C, Mayer JU, Small SJ, Jaimes MC, Price KM. Curr Protoc Cytom. 2020 Mar;92(1):e70. doi: 10.1002/cpcy.70. PMID: 32150355.
Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry
Ferrer-Font L, Small SJ, Lewer B, Pilkington KR, Johnston LK, Park LM, Lannigan J, Jaimes MC, Price KM. Curr Protoc. 2021 Sep;1(9):e222. doi: 10.1002/cpz1.222. PMID: 34492732.